Wolfgang Fecke, Sienna Biotech
Written by Jackie Howard Tuesday, 08 May 2012 10:51
Modulating sphingosine phosphate signaling in recombinant, primary and stem cells – promises and challenges
Although fast and easy to perform, assays with recombinant cell lines and over-expressed proteins do not always reflect the physiological activity of a drug target. Primary cells with relevance to certain diseases are therefore increasingly being used for testing of drug candidates. For instance, modulators of sphingosine phosphate receptors can be profiled with reactive astrocytes which are involved in the development of neuroinflammatory diseases such as multiple sclerosis. Practical issues and data quality using primary and stem cells will be compared to assay performance with conventional cell lines expressing sphingosine phosphate receptors.